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Click “Calculate oxygenation”.
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Oxygenation data
HbO: oxygenated hemoglobin (red by default)
HbR: deoxygenated hemoglobin (blue by default)
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Full screen:
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You can see and modify the color coding in the legend on the right (see bottom here):
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In the main data window, right-click → Display settings:
Data Type: select here which data you want to be displayed
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Data Range: if you have a touch display, you can use your fingers to Zoom or pan, but you can also select a manual range as needed.
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Marker: if you have too many markers, you can select the ones you want to be displayed and not displayed.
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Time: you can select the time window to be displayed as needed, i.e. the range of the x-axis.
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Processing
Creating blocks
In the bottom pane of the main data window, click “Define Blocks”.
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On the left, we define the marker that starts a block and on the right the one that ends that block. Remember, we used an n-back task with several conditions, so we start with the first condition, 0-back.
The button “By Time” and “By Value” are to sort the markers, so you can find them easily in the list. Once you click on a marker, it switches to the “Start pattern” window (same for the “End pattern”).
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We want to define our block from marker 90 to 98 and then check the box “Apply the following label” on the bottom of the right-hand window. We call out blocks “0back” and the click “Run (Step 1)”.
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We now see information in the bottom box saying “Found 7 blocks”. We now click “Save (Step 2)” to save these blocks.
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You can see colored blocks appear on the bottom of our data.
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If you repeat the same process with another condition (In this case 1-back), you also see these markers, leveled slightly differently from the first set.
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If you click “Manage” on the bottom, you see this screen, which shows you all the markers with their block number, onset and offset times, and their duration. You can sort or delete them here.
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Automation
Instead of adding all blocks one by one, we can add all at once with previously configured presets. On the bottom pane, click “Automation”:
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Under NBACK, we have 4 presets saved. Click “Run (Step 1)” and then “Run (Step 2)” and all markers will be added.
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If you click on the available Automation, you will see the details:
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You can then go back to view and edit your blocks by right-click → Manage → Manage Blocks
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Save data
Next, we save the file to the data space: click “Save” in bottom pane.
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Select “All Blocks” → “Next”
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Keep default (I am not sure why this is now raw data instead of refined data or blocks).
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Target location is Data Space:
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A window with a text view of your data will open. This list contains all HBO, HBR and OXY data by block and time.
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If you double-click on a block, you see the data for that block. On the bottom, under Graph Type, you can change the display.
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Click on the tab “Array view…” to see numerical data.
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You can open multiple blocks at the same time to look at them together.
Change baseline
Note that these data have been calculated using the global baseline. We now want to change that. We want to look at the data for the blocks without the influence of the time before the blocks started.
From this window, click on the “Process” tab:
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On the bottom, click “Add/Remove Action”.
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Next, go to Temporal Processing à Conditioning → Correct Baselines and click on the big right arrow to drag the option into the “Selected actions” window. Click “Save”.
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We now see this:
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On the bottom, click “Add/Remove Variables”:
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Select all 28 Oxy blocks and drag them into “Selected Variables”.
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Click “Save”.
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Now you see your process listed. Click “Execute”.
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If you now go back to the Data Space tab, you see a lot of new variables created and they all say “Baseline corrected”. Right-click → view.
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This shows you all averages of HBO for each n-back block for each optode.
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On the bottom, you can change the graph type under and group type. Here 7 trials per block by group (o-back, 1-back, 2-back, 3-back):
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Under Graph type → bar graph, you can see the average across the 7 trials of each block:
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