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  1. Tick the ‘DTI’, ‘fMRI' and ‘T2map’ boxes and click the ‘Preprocess to Coreg’ button. This will create ‘coreg’ folders in each subject’s ‘DTI’, ‘fMRI’ and ‘T2map' folders, with the relevant file in each folder, and a text document file named 'coreg_not_done’.

  2. Go into Realign i.e. coregister the DTI and fMRI images in the ‘coreg’ folders, for each subject, as detailed in steps 10-12 of the confluence page ‘File/Folder Setup and Preparation for Preprocessing’. Write down the pitch, roll, yaw and crosshair position values in the the text document file named ‘coreg_not done’ and rename this file to ‘coreg_*date*'. There is no need to do this for the ‘T2map’ files as they were realgined i.e. coregistered in steps 9-14 from the confluence page ‘File/Folder Setup and Preparation for Preprocessing’.

  3.  

    once you run the ‘Preprocess to Coreg’ steps 10-12, it will create ‘coreg’ folders in each of these foldersNow, go back to the ‘Preclinical’ GUI and unselect the ‘T2map’ box and select the ‘VBM’ box i.e. make sure ‘DTI’, ‘fMRI’ and ‘VBM’ boxes are ticked and that the ‘T2map’ box is unticked.

  4. Then click into the ‘DTI’ (doesn’t exist but it will soon), ‘fMRI’ and ‘VBM’ tabs and set the parameters you desire. E.g.

Screenshot 2024-05-21 120239.jpgImage AddedScreenshot 2024-05-21 120309.jpgImage Added

If you are unsure what value to enter in the ‘Normalised voxel size’ box, open one of your raw T2map images in MRIcron, click ‘Window’ → ‘Information’ and look at the values in the ‘Spacing’ column.

  1. Click the ‘Preprocess after Coreg’ button. This step will take a few hours to complete, depending on how many subjects you have.

  2. Once the ‘Preprocess after Coreg’ step is finished, all of the files necessary for analysis will be in your ‘stats’ folder.

NOTE: For step 6: CHECK WITH PAUL that it is possible to run VBM, DTI and fMRI ‘preprocess after coreg’ all at the same time (once its working for DTI, I think it will be possible to run all at the same time).