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1. From the AcqKnowledge home screen select: Open a graph file, Graph file on Disk, and click OK
   1. This will allow you to access files directly from the U: Drive
      
      
      
2. Minimize all channels except Channels 2 & 9 that correspond to Respiration & ECG respectively, by clicking on the triangle icon on the left hand slide of the channel number.
   
   
   
3. Autoscale waveform horizontally using the autoscale feature in the toolbar
   
   
   
4. The Analysis will identify the R-R interval values in the ECG channel & create a new channel for that waveform. Then it will identify the inspiration & expiration results before conducting the RSA analysis for the Respiration channel
   
   
5. Go to Analysis → HRV and RSA → and select "RSA - Time Series " from the options menu
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6. 
   From the Respiratory Sinus Arrhythmia dialogue menu, set the following settings:
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   1. Click OK
   2. * Consider generating logarithmic RSA for additional analysis
      
      
7. 
   
   Autoscale waveform horizontally using the autoscale feature in the toolbar
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8.