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The "Vertex Analysis: requires you to prepare two sets of files: individual segmented structures combined into one file, and a design matrix and t and f contrasts saved as a set of files. 

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  • .../LinuxShare/FIRST/ID99N20G2/designs/2group_covage/
  • .../LinuxShare/FIRST/ID99N20G2/designs/1group_covagesex/
  • .../LinuxShare/FIRST/ID99N20G2/designs/correlationage/
  • .../LinuxShare/FIRST/ID99N20G2/designs/correlationage_covsex/
  • .../LinuxShare/FIRST/ID99N20G2/designs/regressionageAHI_covsex/
2) Open a terminal in this "design folder"
3) Open Glm
 

Either start from the FSL GUI (if so, open fsl in the design folder), or from a terminal "cd" to the directory, and type Glm (case sensitive).

Example:

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4) Select "Higher-level / non-timeseries design" from the pull-down menu.

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"EV's" at the top of the GUI, not voxel-dependent EV's at the bottom. This will add that number of columns to the design. Do not count the "Group" column in the GUI (the group variable must be pasted as an EV).

7) Click "Paste," then "Clear"

8) In Windows, open the allvariables.txt in Excel. Select the data (not headers) you want to use for the design.

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Ctrl^V does not work, for some odd reason. Right-click does not give a Paste option either. Remember to paste the group variable along with other variables. 

10) Enter the column names.

 

12) Click "Contrasts & F-tests" tab. 

13) Create contrasts:

To compare the means of two samples, set both Contrasts and F-tests to 1. Make sure the box next to C1 is selected (it should be yellow). Select the F1 box. 

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11) Save the file

Under the GLM Setup window click Save, navigate to the directory with the files, and choose a descriptive name ("des_2sample" for example; you can use des_[folder name] using the name of the design folder). You can ignore the warning.

This will save a number of files, as in the example below (where "des" was chosen as the name). These files are used as input to several of the following procedures.

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You will copy the design folder, including all the files, to subfolders of each structure you analyze.

Tip: Glm from FSL GUI

Type "fsl" to start the FSL GUI, click "Misc " then "GLM Setup".

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